The four phytoplankton species toxic M. aeruginosa FACHB942,
toxic A. flos-aquae FACHB-245, C. pyrenoidosa FACHB-9 and
S. obliquus Kütz FACHB-416 were purchased from Institute of Hydrobiology, the Chinese Academy of Sciences. Prior to the initiation
of the experiments, the four phytoplankton species were inoculated
separately into 250 mL sterilized flasks filled with 200 mL
medium (M. aeruginosa and A. flos-aquae: BG-11 medium;
C. pyrenoidosa and S. obliquus: HB-4 medium), and cultured for 7 dayin an incubator with a light intensity of 100 μmol m
−2
s
−1
at 25 ± 1°C
and photoperiod 14:10 h L:D. All the flasks were shaken four times
each day.