10 catfish were randomly sacrificed with
an overdose of MS 222 solution, and triplicate pooled samples were taken for determination
of initial whole body composition, using the same analytical methods used for the
experimental diets. Whole body gross energy content was determined on a Gallenkamp
ballistic bomb calorimeter Loughborough, UK . At the end of the feeding trial, five fish from each dietary treatment n15 fish were analyzed for final body composition and
gross energy