Resistant cultivar 08HN30 carrying the Cf-10 gene was donated by Min Chai (Beijing Vegetable Research Center, Beijing, China), susceptible cultivar 07881 and MoneyMaker which did not contain any known resistance genes (Cf-0) against C. fulvum were maintained in the laboratory (Tomato Research Institute of NEAU, China).
Cultivar 08HN30 was pollinated using pollen from 07881 as paternal to produce F1 plants, F1 plants were self-pollinated to produce F2 mapping population.