Photosystem I and II complexes are both Fe containing proteins, Fe in PS II is important for water splitting (Hulsebosch et al., 1996), thus Fe deficiency may lead to decreased PSII activity.
The activity of PSII was investigated in the present study by using chlorophyll fluorescence technology. The Fv/Fm is a measurement of the light energy transfer in dark adapted samples or the photochemical quantum yield of open PSII centers (DeEll and Toivonen, 2003).