Several studies had been reported regarding the effect of plant growth regulators on callus growth of different plant species .Sirchl et al.found no callus formation from the seed explant of Garcinia mangostana on medium without NAA.According to Satyavathi et al., dicamba was the best plant growth regulator for callus induction from Durum wheat. Callus from Durum wheat was successfully induced at concentration of 2.0 dicamba.kiong et also reported on callus induction from Cycas revoluta, where callus culture was formed on medium supplemented with 20 picloram. 0.5 2,4-D supplemented medium had successfully induced the callus formation from Gymnema sylvestre