Lipid oxidation of the chicken breast samples was determined
using the 2-thiobarbituric acid reactive substances
(TBARS) analysis according to the method of Sinnhuber
and Yu (1977). Each 0.5 g sample was mixed with 3 drops of
antioxidant solution, 3 mL of thiobarbituric acid solution
and 17 mL of 25% (w/v) trichloroacetic acid, followed by
incubation in a water bath at 100C for 30 min. The mixture
was cooled, and a 5 mL volume was removed and centrifuged
at 1,000 x g for 30 min. The absorbance of the supernatant
was measured at 532 nm using a spectrophotometer
(UVmini-1240, Shimadzu, Kyoto, Japan). The results were
expressed as milligram of malonaldehyde (MA) per kilogram
of sample.
Lipid oxidation of the chicken breast samples was determinedusing the 2-thiobarbituric acid reactive substances(TBARS) analysis according to the method of Sinnhuberand Yu (1977). Each 0.5 g sample was mixed with 3 drops ofantioxidant solution, 3 mL of thiobarbituric acid solutionand 17 mL of 25% (w/v) trichloroacetic acid, followed byincubation in a water bath at 100C for 30 min. The mixturewas cooled, and a 5 mL volume was removed and centrifugedat 1,000 x g for 30 min. The absorbance of the supernatantwas measured at 532 nm using a spectrophotometer(UVmini-1240, Shimadzu, Kyoto, Japan). The results wereexpressed as milligram of malonaldehyde (MA) per kilogramof sample.
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