2) Blood test by Test-mate ChE (Model 400), EQM
The Test-mate ChE Cholinesterase Test System is on the basis of Ellman method. Acetylthiocholine (AcTC) or butyrylthiocholine (BuTC) is hydrolyzed by AChE or PChE, producing carboxylic acid and thiocholine, respectively, with reaction to the Ellman reagent (DTNB, dithionitrobenzoic acid) so as to create a yellow color that is gauged spectrophotometrically at 450 nm. The rate of color formation is in proportion to the amount of either AChE or PChE [10]. Nurses collected 20μL of blood per person from both farmers and non-farmers in an air-conditioned room, at the primary health care center, in which optimum temperature was controlled at less than 30 C as recommended by Test-mate ChE Cholinesterase Test System (Model 400) specification. For the farmers, their blood was collected after 24 hours of the last of pesticide application (loading, mixing, and/ or spraying) [11]. And Non-farmer’s blood was collected during their free days. The analysis of cholinesterase level in erythrocyte and plasma using Test-mate was conducted by the researcher.
2) Blood test by Test-mate ChE (Model 400), EQMThe Test-mate ChE Cholinesterase Test System is on the basis of Ellman method. Acetylthiocholine (AcTC) or butyrylthiocholine (BuTC) is hydrolyzed by AChE or PChE, producing carboxylic acid and thiocholine, respectively, with reaction to the Ellman reagent (DTNB, dithionitrobenzoic acid) so as to create a yellow color that is gauged spectrophotometrically at 450 nm. The rate of color formation is in proportion to the amount of either AChE or PChE [10]. Nurses collected 20μL of blood per person from both farmers and non-farmers in an air-conditioned room, at the primary health care center, in which optimum temperature was controlled at less than 30 C as recommended by Test-mate ChE Cholinesterase Test System (Model 400) specification. For the farmers, their blood was collected after 24 hours of the last of pesticide application (loading, mixing, and/ or spraying) [11]. And Non-farmer’s blood was collected during their free days. The analysis of cholinesterase level in erythrocyte and plasma using Test-mate was conducted by the researcher.
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