Ten grams of pepper sarcocarp with 40 mL of 50% ethanol
aqueous were refluxed at 90 C of water bath in 2 h. Then the
extracted solution was collected and the residues were again
extracted with the same conditions until the capsaicinoids were
exhausted. Each solution was used to analyze the content of capsaicinoids
by HPLC.
Then red pigments were further extracted from the residues
after extracting capsaicinoids from 10 g starting material with
40 mL of 95% ethanol for 2 h at 90 C of water bath. The extracted
solution was collected and the residues were again extracted with
the same conditions until the red pigments were exhausted. Each
solution was collected, respectively, and used to determine the
absorbance by spectrophotometric analysis.