The causal fungi,C. musae, Fusariumspp. andL. theobromae, were freshly isolated from infected banana hands. Pure
cultures were maintained on PDA media. Inoculum was prepared by using the conidia of 14-day-old cultures of these
fungi. The conidia were dislodged from the surface of the
media by flooding with sterile distilled water and gentle rubbing
with a sterile glass rod. The suspensions were filtered through
three layers of muslin cloth to remove mycelial fragments and
adjusted to 10
5
conidia/mL with a haemacytometer. Then, all
three conidial suspensions were mixed and adjusted to 5×10
3
conidia/mL