The levels of MUFA were not affected by diet and breed (PN0.05),
this result is not in agreement with the findings of Scollan et al. (2001)
and Choi, Enser, Wood, and Scollan (2000) that showed an increasing
of C18:1 trans, which is a by product of bio-hydrogenation in the
rumen, in meat of animals supplemented with unsaturated FAs.
Whilst IS bulls had higher proportion of C18:2n-6 cis, total PUFAn-
6 and total PUFA, LT from IH bulls contained more C20:2n-6 and
C22:4n-6. Feeding linseed increased the proportion of C18:3n-3,
C20:5n-3 and total PUFAn-3 and decreased the proportion of C20:4n-
6, total PUFAn-6 and PUFAn-6/n-3 ratio. An ordinal breed×diet
interaction from the perspective of the breed factor was observed
(Pb0.05) for PUFA/SFA ratio, which was higher in IS than IH. Choi et al.
(2000) highlighted breed differences in the content of C22:4n-6, but
not in the content of C18:2n-6 of meat phospholipids, however, the
same authors hypothesised genetic differences in de novo FA synthesis
comparing Holstein Frisian and Welsh Blacks steers. Laborde et al.
(2001) have found differences in PUFAn-3, but not in PUFAn-6 and in
total PUFA content comparing the meat of Simmental and Red Angus.
Considering the diet effects, also Maddock et al. (2006) found an