5. ConclusionsThis study reports the different effects of colchicine on in vitro growth and develop- ment of seeds and protocorms of the Brassolaeliocattleya hybrid, from the crossing between Blc. Haw Yuan Beauty × Blc. Goldenzelle “LC”. In addition, we developed an efficient methodology for polyploidization of this orchid of high ornamental value. In addition, the potential of a colchicine-free polyploidization was demonstrated by using individu- alized protocorms as explants. Flow cytometry and cytological analysis were efficient in estimating ploidy and separating polyploid plants from non-polyploid plants.