As the positive control for the PCR assay, three plasmids were constructed by using a TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA, USA). The ITS1 regions of T. rubrum (T99), T. mentagrophytes human-type (T7), and Aspergillus fumigatus (TIMM0108) were cloned into the pCR2.1 plasmid. The copy number of each plasmid was calculated from the total length of the bases and the DNA concentration.