DR0199 protein can bind DNA
To analyse DNA binding by DR0199, we used a random G+C-rich 150 bp duplex DNA (100 pmol) incubated with increasing concentrations (50, 100 and 200 nM) of DR0199 for electrophoretic mobility shift assay. We found that DR0199 (50, 100 and 200 nM) binding duplex DNA formed stable protein–DNA complexes. DNA substrates were incubated with BSA in place of DR0199 as a control. This suggested that DR0199 in D.radiodurans might be a non-specific DNA-binding protein.