The present study was designed to compare egg yolks from three avian species in extender forcryopreservation of Sahiwal bull epididymal spermatozoa. The study was conducted on caudaepididymal spermatozoa from ten slaughtered Sahiwal bulls. Semen retrieved from epididymalcauda of each testes pair was pooled and diluted in tris-citric acid-glycerol extender containing eitherpigeon, guinea fowl or chicken egg yolk semi colon leading to a final spermatozoal concentration of30×106 ml. Diluted semen was transferred into 0.5 ml straws, cooled to 4°C and equilibrated for 4h. The straws were kept on liquid nitrogen vapours for 10 min and then plunged into liquid nitrogenfor storage. Motility, plasma membrane integrity and spermatozoa morphology (acrosome ridge;head, mid piece and tail) of each diluted semen sample were assessed at 0, 2 and 4 h post-thawing.All indicators applied and acrosomal integrity were the highest (P<0.05) after freezing in extendercontaining pigeon egg yolk compared to guinea fowl and chicken egg yolk 0, 2 and at 4 h postthaw.Moreover, spermatozoa tail abnormality percentages were also significantly lower (P<0.05)in extender containing pigeon egg yolk compared to guinea fowl and chicken egg yolk at 0, 2 and 4post-thaw h. In conclusion, pigeon egg yolk used in semen extender improved the post-thaw qualityof Sahiwal bull epididymal spermatozoa.-1KEY WORDS: bulls / cattle / cryopreservation / egg yolk / epididymal spermatozoa / extender / Sahiwal breed / semen /
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