Blind to treatment measurements and scoring of the anatomicalstructures were recorded using Image software (Schneider et al.,2012). First, the scale was set to the number of pixels per millimeter using a 1-mm micrometer. Then, the total body length along thespine and distance between the junctions separating the forebrain,midbrain and hindbrain were measured followed by the area (size)of the swim bladder, area of the pericardial and yolk sac edemawhen present, area and diameter of the optic vesicle (eye), and areaof the otic vesicle. Scores were given to the structures followingspecific criteria