2.2. Bacterial cultures
Escherichia coli JM109, Pseudomonasfluorescens ost5 (16s rRNA
gene sequence submitted to Gen bank, Accession n o. DQ4 39976)
a l a b or at or y i so late , St ap h ylo coc c u s a ur eus ATCC 6538P,Bacillus
cereus MTCC 470, were s tored in 20% glycer ol (v/v) a t 201C.
Before the start of experiments, the cultures w er e gro wn on
n ut rie nt ag ar . T he isola t e s we re su bc ult ur e d t w i c e be for e
inoculation.