2.5.6. Analysis of monoglycerides, diglycerides, triglycerides, free and
total glycerin by GC
For determination of glycerides ASTM D6584 method was used
in a gas chromatograph GC 2010 Shimadzu with on-column injection.
The injection volume was 1
ll. Hydrogen was applied as a carrier
gas. The GC was equipped with a flame ionization detector and
a CG 2010 capillary column (15 m long 0.32 mm internal diameter
0.1 m film thickness) containing 5% phenyl-polydimethylsiloxane
(DB5-HT). The following temperature programme was
applied: 50 C (1 min) – 15 C/min – 180 C–7C/min – 230 C–
30 C/min – 380 C (10 min).