In this paper, a method for measuring caffeine content in
Arabic coffee beans of Ethiopia is reported using UV/vis
spectrophotometer, which is available in most laboratories.
Moreover the methods are easy, fast and cheap for the
determination of the caffeine contents in coffee beans.
The methods include characterizing pure caffeine in water
and dichloromethane, and based on these several techniques
were developed to determine caffeine in coffee beans.
These are extracting caffeine from coffee using dichloromethane
solution. After caffeine is extracted from coffee
using dichloromethane there still exist some interfering
substances from chlorogenic acid related compounds (pcoumaroquinic
acid) that have a maximum peak at a wavelength
of 308–310 nm and these interfering matrices were
eliminated by Gaussian fit.