Production of siderophore
Chrome azurol S (CAS) assay was used to detect siderophores
produced by the selected isolates of EDB (Schwyn and
Neilands 1987). The isolates were stabbed on CAS agar plates
and incubated in the dark at 30 °C for 2 days. After incubation,
the culture plates that changed in color from blue to purple or
yellow were considered as positive for siderophore production.
All experiments were done in five replicates.