A 50 lL sample was incubated with DPPH solutionfor 10 min at room temperature, and DPPH activity was measured by reading the absorbance at 518 nm using a spectrophotometer.
Oxygen radical antioxidant capacity (ORAC) was measured using the Rat ORAC Activity Assay kit (STA-345, Cell Biolabs, Inc. Arjons Drive, USA).
All experiments were repeated three times, and the average value is reported.