In Fig. agarose gel electrophoresis of simplex PCR amplied. land 1, the 16S rDNA gene amplicon (565 bp) of Staph. aureus, lane 2, the hlyA gene amplicon (1412 bp) of L. monocytogenes, lane 3, the eaeA gene amplicon (255 bp) of E. coli O157: H7, lane 4 the invA gene amplicon(786 bp) of Salm. enterica Enteritidis and lane5, the ipaH gene amplicon (1088 bp) of Sh. Flexneri. M was marker and N was negative control
The result showed that the amplification products of the expected sizes were obtained only one amplicon per lane and they were sufficiently different in size to be distinguishable by agarose gel electrophoresis.