The small productivity of enzymes and metabolites from extremophiles represents one of the major bottlenecks in their industrial applications [17]. It has been established that the production of alkaline protease is highly affected by media composition, viz. variation in C/N ratio, and the presence of metabolizable sugars, ions, and salts, in addition to other fermentation parameters including aeration level, pH, temperature, and incubation time [24,25,26]. Hence, it is essential to optimize production medium and cultivation conditions for the growth and enzyme production, in order to obtain high and commercial yields of alkaline protease.