Carrageenan-Induced Granuloma Pouch Model [104].
Carrageenan-induced granuloma pouch model is an excellent
subacute inflammatory model in which fluid extravasations,
leukocyte migration, and various biochemical exudates
involved in inflammatory response can be detected readily.
The air pouch has the advantage of supplying a suitable space
for the induction of inflammatory responses. The injection
of irritants such as carrageenan into subcutaneous air pouch
on the dorsal surface of rats initiates an inflammatory
process. Procedure. The animals used in this method are
rats divided into five groups (n = 6), fasted overnight, and
allowed free access to water. The animals are administered
with vehicle, standard drug, and test drug. One hour after
dosing, the back of the animal is shaved and disinfected. With
a very thin needle, subcutaneous dorsal granuloma pouch
is made in ether anaesthetized rats by injecting 6 mL of air,
followed by injection of 4 mL of 2% carrageenan in normal
saline to avoid any leakage of air and the treatment continued
for seven consecutive days. On day 8, the pouch is opened
under anesthesia and the amount of exudates was collected
with a syringe. The average volume of exudates, total WBC
count, and weights of granuloma is determined.
Carrageenan-Induced Granuloma Pouch Model [104].Carrageenan-induced granuloma pouch model is an excellentsubacute inflammatory model in which fluid extravasations,leukocyte migration, and various biochemical exudatesinvolved in inflammatory response can be detected readily.The air pouch has the advantage of supplying a suitable spacefor the induction of inflammatory responses. The injectionof irritants such as carrageenan into subcutaneous air pouchon the dorsal surface of rats initiates an inflammatoryprocess. Procedure. The animals used in this method arerats divided into five groups (n = 6), fasted overnight, andallowed free access to water. The animals are administeredwith vehicle, standard drug, and test drug. One hour afterdosing, the back of the animal is shaved and disinfected. Witha very thin needle, subcutaneous dorsal granuloma pouchis made in ether anaesthetized rats by injecting 6 mL of air,followed by injection of 4 mL of 2% carrageenan in normalsaline to avoid any leakage of air and the treatment continuedfor seven consecutive days. On day 8, the pouch is openedunder anesthesia and the amount of exudates was collectedwith a syringe. The average volume of exudates, total WBCcount, and weights of granuloma is determined.
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