pineapple puree was individually treated with
different concentrations (0-2.5% v/w, at an interval
of 0.5% v/w) of Pectinex® Ultra SP-L (Novozymes,
Denmark). The enzyme-added purees were incubated
at 50°C and 100 rpm. The TSS, viscosity and pH
of the enzyme liquefied puree were measured at an
interval of 30 minutes for 2.5 hours. The enzyme
in the treated puree was deactivated at 90ºC. The
same procedures were repeated by using Celluclast®
1.5 L (Novozymes, Denmark). Combined enzymes
treatment was then performed by adding the
optimised Pectinex® Ultra SP-L concentration with
Celluclast® 1.5 L at different concentrations (0-
2.5% v/w). The same steps were repeated for the
combination between optimum concentrations of
Celluclast® 1.5 L obtained from single enzyme
treatment with different concentrations of Pectinex®
Ultra SP-L (0-2.5% v/w). Viscosity was chosen as the
key evaluation used to determine the best parameter
for enzyme liquefaction. Liquefied puree with the
lowest viscosity was favourable as it will maximize
the amount of juice yield and prevent the clogging of
spray dryer (Phisut 2012).