Nutritional values (antioxidant activity and vitamin C content), growth and nutrient uptake of Chinese kale seedling in response to high humus seedling media and beneficial microorganisms were evaluated in the screenhouse. Selected seedling media (SMM) were mixed with each of the three selected isolates (Azospirillum sp., Beijerinckia sp. and actinomycetes) and various percentages of leonardite. The results showed that SSM with actinomycetes plus 15 % leonardite produced the highest shoot and root dry weight of Chinese kale seeding and all these values were significantly hiher than the control.