The aim of this molecular study part is to enhance the capacity of the production in wild-type Tetraspora sp. under the optimal condition. Based on the pathway studied in Chlamydomonas reinhardtii, several proteins are involved in biosynthesis pathway of molecular biohydrogen. One of these proteins, sulfate permease (SulP), was selected. Several reports indicated that this protein was embedded on the inner membrane of chloroplast and functioned as the sulfate transporter. Sulfate molecules can be transported from cytoplasm to stroma through this channel. Sulfate will be further used in biosynthesis pathway of cysteine which is a building block for other sulfur-containing amino acids biosynthesis pathway.