Genotyping
Genotyping of the FTO rs8050136 SNP was conducted using the Illumina (San Diego, CA)
GoldenGate chemistry and Sentrix Array Matrix technology on the BeadStation 500GX, using
DNA extracted from leukocytes. For quality control purposes, five CEPH control DNA
samples (NA10851, NA10854, NA10857, NA10860, NA10861; all samples included in the
HapMap Caucasian panel) were genotyped in duplicate. Concordance between the replicates
as well as with the rs8050136 genotypes from the HapMap database was 100%. Findings from
the HapMap have previously shown that rs8050136 and rs9939609 are in very strong linkage
disequilibrium (4).