Each experiment was repeated three times and the data was processed by analysis of variance (ANOVA) using DPS7.05 statistical software (Refine Information Tech. Co., Ltd, Hangzhou, China), comparing treatments at P = 0.05 according to least significant difference (LSD) test, indicating the LSD value in each case. The data values were expressed as mean ± SE (n = 3), where SE is standard error and n is repetition.