Aflatoxin Excretion After Long-Term Exposure
The excretion levels of aflatoxins in both treatment
groups after 21 d of administration were higher than
those obtained in the same animals after short-term exposure
(Figure 1). Recoveries reached 26.9 μg of AFB1
and 37.2 μg of AFM1 in feces and up to 10.7 μg of
AFM1/d in urine. In addition, within each group, less
variation was observed among individuals.
In contrast to the short-term exposure, after the 21-d
exposure a clear positive effect of YCW was observed
on fecal excretion patterns (Table 4). The presence
of AFB1 and AFM1 was increased in the feces of the
AF+YCW group (P < 0.05), particularly for AFM1,
which increased up to 100% compared with the AF
group (P < 0.05). This higher excretion of aflatoxins
in feces was concomitant with a lower concentration of
AFM1 in urine samples compared with concentrations
observed in the AF group, although the differences were
not significant. In contrast, YCW did not affect the
level of AFM1 in milk (Table 3). Concentration profiles
of AFM1 in milk and transfer parameters did not differ
between AF and AF+YCW groups (P > 0.05).