MOBILE PHASE: 250 mM (NH4)2SO4 /100 mM NH4OH
FLOW RATE: 1.0 mL/min; 0.7 mL/min post column
POST-COLUMN REAGENT: 2.0 mM DPC + 10% MeOH +1N H2SO4
DETECTOR: 540 nm
CALIBRATION: Standard solutions of K2Cr2O7 in NaOH-Na2CO3 buffer
RANGE: 0.05 to 20 :g per sample
ESTIMATED LOD: 0.02 :g per sample [3]
PRECISION (þr): 0.015 @ 0.5 to 5 :g/sample [3]