3.3. Effect of P. betle extract on inhibition and eradication biofilm
formation
The inhibition and eradication of the biofilm formation of P. betle
extract against S. mutans ATCC 25175 and A. actinomycetemcomitansATCC 33384 is demonstrated in Fig. 2a and b, respectively. The
concentrations of P. betle extract required to inhibit for 50% biofilm
formation (MBIC50) of S. mutans ATCC 25175 and
A. actinomycetemcomitans ATCC 33384 were 0.39 ± 0.20 and0.10 ± 0.03 mg/mL, respectively. And for 90% biofilm inhibitions
(MBIC90) were 1.56 ± 0.11 and 0.39 ± 0.65 mg/mL, respectively. The
concentrations of P. betle extract to eradicate for 50% biofilm
formation (MBEC50) of S. mutans ATCC 25175 and
A. actinomycetemcomitans ATCC 33384 were 0.78 ± 0.74 and
0.78 ± 0.11 mg/mL, respectively. And for 90% biofilm eradication
(MBEC90) were 6.25 ± 0.58 and 3.13 ± 0.28 mg/mL, respectively.
CHX could completely (100%) inhibit or remove biofilm formation
of both tested strains.