then applied onto a MALDI plate and allowed to dry at room temperature. ForPMF analysis spectrometer was operated in the reflectron mode,m/z range 700–3500, the laser power was 70 and 100 sam-ple profiles were acquired, where each profile was the averageof 20 laser shots. For calibration PeptideMass Calibration Kit(Sigma, Cat. No-1KT MSCAL2) was used. The spectra were pro-cessed with the program Axima Biotech Launchpad (Shimadzu,Japan).2.5.