The total tannin content in the lyophilized plant extract was determined by modification of a previous method (Polshettiwar et
al., 2007).
The sample (0.1 ml) was mixed with 0.5 ml of Folin-Denis reagent followed by 1 ml of Na2CO3 (0.5% w/v) solution and
distilled water (up to 5 ml).
The absorbance was measured at 755 nm within 30 min of the reaction against the reagent blank.
Total tannins in extract were expressed as equivalent to tannic acid (gTE/g extract).