Furthermore, when using cel-miR-39 for normalization we
found significantly higher levels (Po0.05) of all investigated
miRNAs in the six biobank A2 samples (1.9–368 fold on average)
and of all investigated miRNAs except miR-26a in the biobank B2
samples (1.6–97.4 fold on average) as compared to PPP samples.
Alongside that, miRNA levels normalized to miR-16 were found to
be significantly higher (po0.05) for 10 of the investigated miRNAs
in biobank A2 samples (2.3–233 fold on average) and for all of
the investigated miRNAs except miR-26a in biobank B2 samples
(1.4–53.6 fold on average). The level of miR-30a-5p and miR-30d
were found to be higher (1.4–2.2 fold on average), but not significantly different between biobank A2 and PPP samples
(p40.05). Levels of miR-26a were found to be slightly lower in
biobank A2/B2-samples compared to PPP, and in A2 samples the
difference was not significant.