. The system was programmed in steps of 0–18% B over 15 min, maintenance of 18% B for 3 min, return to the initial conditions over 1 min, and equilibration for 4 min. The mobile phase was continuously sparged with nitrogen. T
. The system was programmed in steps of 0–18%B over 15 min, maintenance of 18% B for 3 min, return to the initial conditionsover 1 min, and equilibration for 4 min. The mobile phase wascontinuously sparged with nitrogen. T