Measurement of vitamin-C content
The 2, 6-dichlorophenolindophenol method was used to assay vitamin C content in juice by
spectrofluorometer. A wavelength of 350–430 nm was used to determine total vitamin C content
[8]. For the measurement of vitamin-C content, reagents used were 5% metaphosphoric acid
solutions, ascorbic acid standard solution, 0.025% 2, 6-dichlorophenol indophenol reagent.