The gelatin medium prepared and sterilized by tyndallization,
keeping the medium in steaming for 3 consecutive days. Then the
medium was inoculated heavily with the test organism by the stab
inoculation and incubated for 96 h. After the incubation period the
hydrolysis of the gelatin was indicated by the liquefaction of
gelatin. The culture was incubated at room temperature and chilled
until the control solidifies before observations of liquefactions were
made