A 500 μM stock solution of Pep-1 was prepared by dissolution in
18 MΩ water and was then stored as 5 μL aliquots in lo-bind eppendorf
tubes at −20 °C. A 50 μM stock solution of HRP was prepared by dissolution
in 18 MΩ water and was then stored as aliquots at −20 °C.
50 mM Amplex Red was prepared by dissolution in DMSO and stored
at−4 °C. Pep-1/HRP complexes were prepared fresh fromthe stock solutions
prior to each experiment