The host strain is designed as CodonPlus E. coli strain of ‘Stratagene’ (RIL or RP) and Rosetta strain (Novagene). Rosetta host strains are BL21(DE3) derivatives designed to enhance the expression of heterologous proteins by containing codons rarely used in E. coli. CodonPlus-RIL strain is used to overcome the bias of AT-rich genome while CodonPlus-RP strain is used to overcome GC rich genome bias.