Three species of Aspergillus genera were cultured in
Adams, Czapeck, Khanna, M5 and Vogel culture media.
Adams medium was more favorable for enzyme production
for Aspergillus japonicus 246A (19.8 U/mg) and
Aspergillus sp. GM4 (12.0 U/mg) (Table 3), followed by
Khanna and Czapeck. M5 medium was not favorable for
tannase production, which could have been due to the
composition of the medium, with yeast extract and peptone
as additional carbon sources. A. tamarii 3 presented
a lower tannase activity compared to the other species,
with a maximum production of 3.0 U/mg in Czapeck
medium. Protein content of and tannase production by
Aspergillus japonicus 246A and Aspergillus sp. GM4
were very similar. Many studies have been conducted
with A. japonicus for tannase production. Therefore, we
selected Aspergillus sp. GM4 for optimization of tannase
production culture conditions.