2.2.2. Test Procedure of Total Aflatoxins
According to Ridascreen Aflatoxin Total (Art no.: 4701) test kit manual [23], 50 μL of the standard solutions or prepared sample in duplicate were added to the wells of microtiter plate. Then 50 μL of the diluted enzyme conjugate and 50 μL of the diluted antibody solution were added to each well