HepG2 is a perpetual cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15-year-old Caucasian male who had a well-differentiated hepatocellular carcinoma. Hepatocellular carcinoma is, worldwide, the fifth most-common cancer. The morphology of HepG2 cells is epithelial and they have 55 chromosome pairs. HepG2 cells can be grown successfully at a large scale, and secret many plasma proteins, such as transferring, fibrinogen, plasminogen and albumin. They can be stimulated with human growth hormone. HepG2 are adherent, epithelial-like cells growing as monolayers and in small aggregates. Not tumorigenic in immunocompromised mice.