What aspect of DNA polymerase function necessitates use of telomerase in eukaryotes?
A) 3'-5' exonuclease function.
B) 5'-3' processivity.
C) Induced fit impact on incorporation of only correct bases.
D) All of these.
E) None of these.
Telomerase activity in humans is generally restricted to the germ line, cancerous cells, and a few specific adult cell types. How would this be expected to impact humans?
A) Cancer cells can divide indefinitely.
B) Because there is a difference in number of divisions, sperm would be expected to have shorter telomeres than oocytes.
C) Rapidly dividing populations of cells have telomeres equal in size to slowly dividing populations of cells.
D) All of these.
E) None of these.
Base pairing rules are critical for the ability of each DNA strand to act as a template.
A) True
B) False
Meselson and Stahl's experiment was able to demonstrate in one round of division that DNA replication is a semiconservative process.
A) True
B) False
DNA replication requires an origin, where the two strands can be separated.
A) True
B) False
DnaA protein is unable to bind hemimethylated DNA because its binding site includes a GATC methylation site.
A) True
B) False
DNA replication proceeds only in one direction.
A) True
B) False
DNA polymerase recognizes correct base pairs and incorporates new nucleotides which are correctly base paired to the template.
A) True
B) False
The fidelity of DNA replication in E. coli is about one mistake per 100 million incorporated nucleotides.
A) True
B) False
Fig 11.7 is an accurate depiction of the relative position of the enzymes required for DNA replication.
A) True
B) False
DNA polymerase moves toward the replication fork on both the lagging and leading strands.
A) True
B) False
DNA polymerase uses energy contained in the linkage between phosphate groups to drive DNA synthesis.
A) True
B) False
After DNA replication, the newly synthesized strand contains only newly synthesized histones.
A) True
B) False