4 min at 70uC, 4 cycles of 2 min at 94uC, 30 s at 51uC, and
1–3 min at 72uC, followed by 36 cycles of 30 s at 94uC, 30 s at
53uC, and 1–3 min at 72uC, with a final elongation for 10 min at
72uC; for the nuclear genes, 4 min at 70uC, 4 cycles of 2 min at
94uC, 30 s at 57uC, and 5 min at 68uC, followed by 36 cycles of
30 s at 94uC, 30 s at 60uC, and 5 min at 68uC, with a final
extension for 15 min at 68uC