FAMEs n-hexane (1μL) was injected into the
column using a Varian 8200 CX Autosampler. The injection port and detector were both maintained at 250 °C. Hydrogen, at 45 psi, functioned
as the carrier gas, while nitrogen was employed as the makeup gas.
Varian Star Chromatography Software recorded the chromatograms.
Fatty acid methyl ester samples were identified by comparing the
retention times of FAME peaks from samples with those of standards
obtained from Supelco (Supelco 37 Component Fame Mix 47885-U,
Sigma-Aldrich Aston Manor, Pretoria, South Africa). Conjugated
linoleic acid (CLA) standards were obtained from Matreya Inc. (Pleasant
Gap, Unites States).