Detailed extraction conditions of temperature and concentration
of ethanol are shown in Table 1. Ground samples (2 g) were
extracted with 20 ml of the corresponding aqueous ethanol
concentration at the temperature pre-established in the factorial
design using a Soxtec System HT (1043 – Tecator). Subsequently,
the extract was dehydrated to obtain a dry extract and diluted with
ethanol up to 20 mg/ml of extract concentration. After that, the extract
was centrifugated at 16,249g for 5 min, and the supernatant
was used for the antioxidant determination.