Carbohydrates were analyzed as monosaccharides, according to NREL/TP-510-42623. Each substrate was homogenized prior to sampling. Samples were diluted 10x, acidified with 4% H2SO4, and autoclaved at 121ºC for 1 h to hydrolyze polysaccharides. Samples were neutralized with CaCO3, and then analyzed Via HPLC with an Aminex HPX-87P Column and a refractive index detector. Sugar recovery standards were analyzed to correct for loss of monosaccharides during acid hydrolysis. Net digestion results were corrected for dilution due to 2N NaOH addition during digestion.