To detect Staphylococcus aureus we studied a small black and shiny colony with oil zone, indicating Staphylococcus aureus. A slide was prepared from an isolated colony and Gram staining method was applied. For final verification of Staphylococcus aureus, citrated plasma was used to investigate the coagulase. S ,aureus catalase test was positive, ability to ferment mannitol tests ,DNase was posetive, hemolytic zone on 5% sheep blood agar and VP test were also performed.