Stock การเพาะเลี้ยง of the isolates were stored in De Man, Rogosa and Sharpe (MRS) broth medium for LAB or Brain Heart Infusion (BHI) for E. coli and B. subtilis, containing 40% glycerol (Carlo Erba, Italy) and kept at − 80°C. For routine cultivation, the strains were grown overnight in MRS broth or BHI at 30°C. All strains were inoculated at 10% (v/v).