All egg injections were performed at 18.5 doi (corresponded to 18.5 or 19.0 IAN because of 12 h difference in time of set) using a commercial Embrex Inovoject multi-egg injection system Eggs were injected through the air cell with a blunt tipped injector needle to target the amnion. The needle provided an approximate 2.49 cm injection depthfrom the top of the large end of the egg. As previously described by Sokale et al